Troubleshooting with the Grid/Collection Stitching plugin Inversion of CoordinatesĬertain data sets have caused an inversion of coordinates when stitched together. Click ok and the images will begin to be stitched together.Alternatively, more memory can be allocated to the Fiji operation.If the data computed is too large for computer memory, check “use virtual memory.” This will be considerably slower, but will save RAM.Reduce “Increase overlap” to 0 and uncheck subpixel accuracy.if Prairie or WiscScan are used, uncheck “compute overlap.” Both softwares calculate fairly accurate coordinates beforehand.To learn about other fusion methods, please visit the Stitching page.To save time, only use linear blending for presentation pieces. Without it, tile lines will be clearly seen if the image is to be blown up. Linear blending will obscure the seams between tiles, but will take a longer amount of time.If Prairie was used for data collection, ensure that the file selected is a.When a second GUI appears, select a file from your dataset.With this choice, the images within your file do not need to be in any particular order. Then choose the order as Defined by image metadata. In the plugin’s graphical user interface (GUI), choose the position type as Positions from file from the first dropbox menu.Launch the stitching plugin from the menu item Plugins › Stitching › Grid/Collection stitching.How to use the Grid/Collection Stitching plugin The Grid/Collection stitching plugin allows several tiles placed in varying dimensions to be stitched together. Some overlap will be present between each tile and its neighbors to verify its location in the image. Once the entire specimen has been imaged, the tiles must be fit or stitched together to form one coherent image. When a large biological specimen must be pictured in high resolution, it must be done in tiles as the entire thing could not fit into the field view of the microscope.
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